01755nas a2200217 4500008004100000020001400041245010500055210006900160260003900229300001400268490000800282520108300290653001601373653001801389653001301407653001701420653001201437100002501449700002301474856004001497 2024 eng d a1552-500700aA review of the reproductive biology of mormyroid fishes: An emerging model for biomedical research0 areview of the reproductive biology of mormyroid fishes An emergi bJohn Wiley & Sons, Ltdc2024/05/01 a144 - 1630 v3423 aAbstract Mormyroidea is a superfamily of weakly electric African fishes with great potential as a model in a variety of biomedical research areas including systems neuroscience, muscle cell and craniofacial development, ion channel biophysics, and flagellar/ciliary biology. However, they are currently difficult to breed in the laboratory setting, which is essential for any tractable model organism. As such, there is a need to better understand the reproductive biology of mormyroids to breed them more reliably in the laboratory to effectively use them as a biomedical research model. This review seeks to (1) briefly highlight the biomedically relevant phenotypes of mormyroids and (2) compile information about mormyroid reproduction including sex differences, breeding season, sexual maturity, gonads, gametes, and courtship/spawning behaviors. We also highlight areas of mormyroid reproductive biology that are currently unexplored and/or have the potential for further investigation that may provide insights into more successful mormyroid laboratory breeding methods.10abiomedicine10aelectric fish10amormyrid10areproduction10ateleost1 aSaunders, Alyssa, N.1 aGallant, Jason, R. uhttps://doi.org/10.1002/jez.b.2324203202nas a2200301 4500008004100000022001400041245015100055210006900206260000900275300001300284490000700297520224500304653001202549653001802561653001902579653001602598653002002614653003002634653003102664653001402695653003602709653002402745653001802769100001802787700002202805700002102827856005202848 2020 eng d a1932-620300aTranscriptome-wide single nucleotide polymorphisms related to electric organ discharge differentiation among African weakly electric fish species.0 aTranscriptomewide single nucleotide polymorphisms related to ele c2020 ae02408120 v153 a

African weakly electric fish of the mormyrid genus Campylomormyrus generate pulse-type electric organ discharges (EODs) for orientation and communication. Their pulse durations are species-specific and elongated EODs are a derived trait. So far, differential gene expression among tissue-specific transcriptomes across species with different pulses and point mutations in single ion channel genes indicate a relation of pulse duration and electrocyte geometry/excitability. However, a comprehensive assessment of expressed Single Nucleotide Polymorphisms (SNPs) throughout the entire transcriptome of African weakly electric fish, with the potential to identify further genes influencing EOD duration, is still lacking. This is of particular value, as discharge duration is likely based on multiple cellular mechanisms and various genes. Here we provide the first transcriptome-wide SNP analysis of African weakly electric fish species (genus Campylomormyrus) differing by EOD duration to identify candidate genes and cellular mechanisms potentially involved in the determination of an elongated discharge of C. tshokwe. Non-synonymous substitutions specific to C. tshokwe were found in 27 candidate genes with inferred positive selection among Campylomormyrus species. These candidate genes had mainly functions linked to transcriptional regulation, cell proliferation and cell differentiation. Further, by comparing gene annotations between C. compressirostris (ancestral short EOD) and C. tshokwe (derived elongated EOD), we identified 27 GO terms and 2 KEGG pathway categories for which C. tshokwe significantly more frequently exhibited a species-specific expressed substitution than C. compressirostris. The results indicate that transcriptional regulation as well cell proliferation and differentiation take part in the determination of elongated pulse durations in C. tshokwe. Those cellular processes are pivotal for tissue morphogenesis and might determine the shape of electric organs supporting the observed correlation between electrocyte geometry/tissue structure and discharge duration. The inferred expressed SNPs and their functional implications are a valuable resource for future investigations on EOD durations.

10aAnimals10aelectric fish10aelectric organ10aElectricity10aGene Expression10aGene Expression Profiling10aGene Expression Regulation10aPhenotype10aPolymorphism, Single Nucleotide10aSpecies Specificity10atranscriptome1 aCanitz, Julia1 aKirschbaum, Frank1 aTiedemann, Ralph uhttps://mormyrids.efishgenomics.com/en/node/62601997nas a2200229 4500008004100000022001300041245008100054210006900135260001600204520128400220653001101504653001801515653001901533653001301552653002001565653001601585100002601601700002901627700002201656700002301678856006601701 2016 eng d a0928425700aElectric Fish Genomics: Progress, Prospects, and New Tools for Neuroethology0 aElectric Fish Genomics Progress Prospects and New Tools for Neur cJan-10-20163 a

Electric fish have served as a model system in biology since the 18th century, providing deep insight into the nature of bioelectrogenesis, the molecular structure of the synapse, and brain circuitry underlying complex behavior. Neuroethologists have collected extensive phenotypic data that span biological levels of analysis from molecules to ecosystems. This phenotypic data, together with genomic resources obtained over the past decades, have motivated new and exciting hypotheses that position the weakly electric fish model to address fundamental 21st century biological questions. This review article considers the molecular data collected for weakly electric fish over the past three decades, and the insights that data of this nature has motivated. For readers relatively new to molecular genetics techniques, we also provide a table of terminology aimed at clarifying the numerous acronyms and techniques that accompany this field. Next, we pose a research agenda for expanding genomic resources for electric fish research over the next 10 years. We conclude by considering some of the exciting research prospects for neuroethology that electric fish genomics may offer over the coming decades, if the electric fish community is successful in these endeavors.

10aCRISPR10aelectric fish10agenome editing10agenomics10atranscriptomics10atransgenics1 aPitchers, William, R.1 aConstantinou, Savvas, J.1 aLosilla, Mauricio1 aGallant, Jason, R. uhttp://linkinghub.elsevier.com/retrieve/pii/S092842571630007903504nas a2200349 4500008004100000020001400041245015000055210006900205260000800274300001400282490000700296520244700303653002302750653000902773653001802782653002902800653003602829653001802865653001202883653002202895653001602917653001402933653002502947653002702972653001502999653001403014100002403028700002203052700002503074700002103099856003403120 2008 eng d a1010-061X00aDifferentiation of morphology, genetics and electric signals in a region of sympatry between sister species of African electric fish (Mormyridae)0 aDifferentiation of morphology genetics and electric signals in a cJul a1030-10450 v213 a

Mormyrid fishes produce and sense weak electric organ discharges (EODs) for object detection and communication, and they have been increasingly recognized as useful model organisms for studying signal evolution and speciation. EOD waveform variation can provide important clues to sympatric species boundaries between otherwise similar or morphologically cryptic forms. Endemic to the watersheds of Gabon (Central Africa), Ivindomyrus marchei and Ivindomyrus opdenboschi are morphologically similar to one another. Using morphometric, electrophysiological and molecular characters [cytochrome b sequences and amplified fragment length polymorphism (AFLP) genotypes], we investigated to what extent these nominal mormyrid species have diverged into biological species. Our sampling covered the known distribution of each species with a focus on the Ivindo River, where the two taxa co-occur. An overall pattern of congruence among datasets suggests that I. opdenboschi and I. marchei are mostly distinct. Electric signal analysis showed that EODs of I. opdenboschi tend to have a smaller initial head-positive peak than those of I. marchei, and they often possess a small third waveform peak that is typically absent in EODs of I. marchei. Analysis of sympatric I. opdenboschi and I. marchei populations revealed slight, but significant, genetic partitioning between populations based on AFLP data (F-ST approximate to 0.04). Taken separately, however, none of the characters we evaluated allowed us to discriminate two completely distinct or monophyletic groups. Lack of robust separation on the basis of any single character set may be a consequence of incomplete lineage sorting due to recent ancestry and/or introgressive hybridization. Incongruence between genetic datasets in one individual, which exhibited a mitochondrial haplotype characteristic of I. marchei but nevertheless fell within a genetic cluster of I. opdenboschi based on AFLP genotypes, suggests that a low level of recent hybridization may also be contributing to patterns of character variation in sympatry. Nevertheless, despite less than perfect separability based on any one dataset and inconclusive evidence for complete reproductive isolation between them in the Ivindo River, we find sufficient evidence to support the existence of two distinctive species, I. opdenboschi and I. marchei, even if not ’biological species’ in the Mayrian sense.

10aadaptive radiation10aaflp10aelectric fish10aelectric organ discharge10agnathonemus-petersii mormyridae10aintrogression10amarkers10amitochondrial-DNA10amormyroidea10aphylogeny10apopulation-structure10areproductive isolation10aspeciation10ateleostei1 aLavoué, Sébastien1 aSullivan, John, P1 aArnegard, Matthew, E1 aHopkins, Carl, D u://00025668710001000736nas a2200193 4500008004100000245020600041210006900247260001200316300001400328653000900342653001800351653001900369653002000388653001800408100002300426700002200449700002100471856005000492 2014 eng d00aDe novo assembly and characterization of the skeletal muscle and electric organ transcriptomes of the African weakly-electric fish Campylomormyrus compressirostris (Mormyridae, Teleostei)0 aiDe novoi assembly and characterization of the skeletal muscle a c03/2014 an/a - n/a10acSSR10aelectric fish10aelectric organ10askeletal muscle10atranscriptome1 aLamanna, Francesco1 aKirschbaum, Frank1 aTiedemann, Ralph uhttps://mormyrids.efishgenomics.com/en/node/8