<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Baumann, Otto</style></author><author><style face="normal" font="default" size="100%">Cheng, Feng</style></author><author><style face="normal" font="default" size="100%">Kirschbaum, Frank</style></author><author><style face="normal" font="default" size="100%">Tiedemann, Ralph</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Organization of the stalk system on electrocytes in mormyrid weakly electric fish Campylomormyrus compressirostris</style></title><secondary-title><style face="normal" font="default" size="100%">Cell and Tissue Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ca2+-ATPase</style></keyword><keyword><style  face="normal" font="default" size="100%">Confocal microscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">electrocyte</style></keyword><keyword><style  face="normal" font="default" size="100%">F-actin</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasma membrane</style></keyword><keyword><style  face="normal" font="default" size="100%">Sodium pump</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Dec-20-2024</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://doi.org/10.1007/s00441-024-03938-y</style></url></web-urls></urls><pages><style face="normal" font="default" size="100%">1-17</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;jats p=&quot;&quot;&gt;The adult electric organ in weakly electric mormyrid fish consists of action-potential-generating electrocytes, structurally and functionally modified skeletal muscle cells. The electrocytes have a disc-shaped portion and, on one of its sides, numerous thin processes, termed stalklets. These unite to stalks leading to a single main stalk that carries the innervation site. Here, we describe the 3-dimensional layout of the stalklet/stalk system in adult &lt;jats italic=&quot;&quot;&gt;&lt;em&gt;Campylomormyrus compressirostris&lt;/em&gt;&lt;/jats&gt; by differential interference contrast microscopy and confocal fluorescence microscopy. Using antibodies against Na&lt;jats sup=&quot;&quot;&gt;+&lt;/jats&gt;/K&lt;jats sup=&quot;&quot;&gt;+&lt;/jats&gt;-ATPase &amp;alpha;-subunit and plasma membrane Ca&lt;jats sup=&quot;&quot;&gt;2+&lt;/jats&gt;-ATPase, we show that these ion pumps are differentially distributed over the stalklet/stalk system, with plasma membrane Ca&lt;jats sup=&quot;&quot;&gt;2+&lt;/jats&gt;-ATPase being enriched on the stalklet membrane. Stalklets are distributed and organized in a quite uniform pattern on the posterior face of the electrocyte disc and fuse to terminal stalks. The latter then unite in a mostly dichotomic mode to stalks of increasing thickness, with the main stalk measuring about 100&amp;nbsp;&amp;micro;m in diameter. We further analyse the structural organization of stalklets and stalks, with a characteristic cytoskeletal system of bundled actin filaments in the centre and nuclei in subsurface position. These results suggest that the stalklet/stalk system is adapted in its structural layout to generate an action potential highly synchronized over the entire disc-portion of the electrocyte, accounting for the short electric organ discharge in this species. Our results suggest that actin-related proteins overexpressed in electrocytes, as shown previously by transcriptome analysis, may be involved in the organization of the unique F-actin system in stalklets and stalks.&lt;/jats&gt;&lt;/p&gt;
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